… Therefore, Okazaki fragments are formed during the synthesis of lagging template strand. Hence, both strands should serve as a template in DNA replication. Why do Okazaki fragments form during DNA replication?ĭNA replication occurs in a semi-conservative manner where one of the DNA strands in the newly synthesized double-stranded DNA is an original strand. Why must there be a lagging strand during DNA synthesis? Explanation: The lagging strand exists because DNA is antiparallel and replication always occurs in the 5′ to 3′ direction. It only replicates continuously (like the leading strand), and uses DNA primers. PCR doesn’t have a leading and lagging strand, nor does it use RNA primers, okazaki fragments, etc. On the lagging strand, DNA synthesis restarts many times as the helix unwinds, resulting in many short fragments called “Okazaki fragments.” DNA ligase joins the Okazaki fragments together into a single DNA molecule. Okazaki fragments are short sequences of DNA nucleotides (approximately 150 to 200 base pairs long in eukaryotes) which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication. What happens during the formation of Okazaki fragments quizlet? … During the process of DNA replication, DNA and RNA primers are removed from the lagging strand of DNA to allow Okazaki fragments to bind to. Newly synthesized DNA, otherwise known as Okazaki fragments, are bound by DNA ligase, which forms a new strand of DNA. What happens during the formation of Okazaki fragments? … DNA polymerase catalyzes the addition of nucleotides to a growing DNA chain. Okazaki fragments are short sequences synthesized in the lagging strand because DNA polymerase can synthesize only from 5′ to 3′, and the DNA strands are antiparallel. How are Okazaki fragments synthesized quizlet? Okazaki fragments are formed on the lagging strand for the synthesis of DNA in a 5′ to 3′ direction towards the replication fork. Okazaki fragments are formed on lagging strands, initiated by the creation of a new RNA primer by the primosome.
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